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sequencing libraries (poly-a capture, non-directional library preparation)  (Novogene)

 
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    Structured Review

    Novogene sequencing libraries (poly-a capture, non-directional library preparation)
    A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition <t>(TIDE)-sequencing</t> (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.
    Sequencing Libraries (Poly A Capture, Non Directional Library Preparation), supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing libraries (poly-a capture, non-directional library preparation)/product/Novogene
    Average 90 stars, based on 1 article reviews
    sequencing libraries (poly-a capture, non-directional library preparation) - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Riboflavin drives nucleotide biosynthesis and iron-sulfur metabolism to promote acute myeloid leukemia"

    Article Title: Riboflavin drives nucleotide biosynthesis and iron-sulfur metabolism to promote acute myeloid leukemia

    Journal: bioRxiv

    doi: 10.1101/2025.06.26.661633

    A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition (TIDE)-sequencing (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.
    Figure Legend Snippet: A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition (TIDE)-sequencing (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.

    Techniques Used: Recombinant, Sequencing



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    Novogene sequencing libraries (poly-a capture, non-directional library preparation)
    A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition <t>(TIDE)-sequencing</t> (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.
    Sequencing Libraries (Poly A Capture, Non Directional Library Preparation), supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing libraries (poly-a capture, non-directional library preparation)/product/Novogene
    Average 90 stars, based on 1 article reviews
    sequencing libraries (poly-a capture, non-directional library preparation) - by Bioz Stars, 2026-06
    90/100 stars
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    A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition (TIDE)-sequencing (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.

    Journal: bioRxiv

    Article Title: Riboflavin drives nucleotide biosynthesis and iron-sulfur metabolism to promote acute myeloid leukemia

    doi: 10.1101/2025.06.26.661633

    Figure Lengend Snippet: A . CD34 + HSPC cells nucleofected with recombinant Cas9 and sgRNAs targeting Rosa or with two independent sgRNAs targeting RFK for 15 days. Colony formation capacity was determined (left) and colony number was quantified (middle). Effective editing of RFK by the two sgRNAs was determined using Tracking of Indels by Decomposition (TIDE)-sequencing (right). B . MOLM-13 cells were treated with escalating concentrations of venetoclax and roseoflavin for 72 hours to determine viability effects. The presence of treatment synergy was determined using SynergyFinder and the Bliss synergy index and is denoted as regions of red in the graphs. The mean of three biological replicates was used for each data point. Data are presented as the mean ± SD. **** P < 0.0001 by ordinary one-way ANOVA with Bonferroni’s multiple comparisons test.

    Article Snippet: Sequencing libraries (poly-A capture, non-directional library preparation) were generated by Novogene according to the manufacturer’s protocol, and were sequenced on the Illumina NovaSeq 6000, with paired end 150 bp reads (Q30 ≥ 85%) to a depth of 20 million reads per sample.

    Techniques: Recombinant, Sequencing